Curs de  Citometrie pentru incepatori

Beginners – Flow Cytometry

SPITALUL UNIVERSITAR DE URGENTA BUCURESTI

EMERGENCY UNIVERSITY HOSPITAL BUCHAREST

 

 

Speakers:

 

Claude Lambert This email address is being protected from spambots. You need JavaScript enabled to view it.

Eugen Radu This email address is being protected from spambots. You need JavaScript enabled to view it.

Magda Mocanu This email address is being protected from spambots. You need JavaScript enabled to view it.

Horia Bumbea This email address is being protected from spambots. You need JavaScript enabled to view it.

Bianca Gălățeanu This email address is being protected from spambots. You need JavaScript enabled to view it.

Luminita Marutescu This email address is being protected from spambots. You need JavaScript enabled to view it.

Ion Dumitru This email address is being protected from spambots. You need JavaScript enabled to view it. - aplicatii practice / practical

 

ZIUA 1 / DAY 1

17 Oct 2017

13rd floor

                      

 

08.30-09.00

 

INREGISTRAREA PARTICIPANTILOR /  REGISTRATION

 

 

 

09.00-10.00

Prezentare generala a cursului

Principii ale CMF

Aplicatii, Sistem fluidic, Sistem optic, Sistem electronic, Tipuri de instrumente

 

Course overview

FCM principles

Applications, fluidics, optics, electronics, types of instruments

 

 

10.00-11.00

Fluorescenta

Principii fluorescenta, Fluorocromi, Markeri tandem, Interferenta, Selectia filtrelor. Sistem optic

 

Fluorescence

Principle of fluorescence, fluorochromes, tandem dyes, interference, filter selection Optical bench

 

 

11.00-11.30

 

Coffee Break

 

11.30-12.30

Design experimental. Preparare probe, achizitie, analiza date

Achizitie, prezentare, analiza si interpretare

 

Experimental design. Sample preparation, acquisition, Data analysis

Acquisition, display, analysis and interpretation

 

 

12.30-14.30

 

Prânz / Lunch

 

 

 

APLICAȚII PRACTICE / PRACTICAL

ETAJ 13 - LABORATOR

13rd FLOOR - LABORATOR

 

14.30-16.00

Group 1 Instrument show; sample preparation, analysis (prepare samples 6 colors for day 2)

 

Group 2 Computer-excercises: Compensation settings

 

16.00-16.30

 

Coffee Break

 

 

 

16.30-18.00

Group 2 Instrument show; sample preparation, analysis (prepare samples 6 colors for day 2)

 

Group 1 Computer-excercises: Compensation settings

 

 

 

 

 

 

 

19:00 - 22:00

 

 

Cina / Dinner

 

               ZIUA 2 / DAY 2ound floor

18 Oct

13rd floor

 

 

09.00-10.00

Aplicatii ale CMF in biologia celulara (C1)

Ciclu celular, Viabilitate

 

FCM applications in cell biology

Cell cycle, viability

 

 

10.00-11.00

Aplicatii ale CMF in biologia celulara

Fenotipuri. Citometrie cantitativa

FCM applications in cell biology  phenotypes, quantitative cytometry

 

 

11.00-11.30

 

Coffee Break

11.30-13.00

Detectia fluorescentei, setari instrumente, compensari,. Design protocol

 

Fluorescence detection, instrument settings, compensations. Protocol design

 

 

 

12.30-14.30

 

Pranz / Lunch

 

 

APLICATII PRACTICE / PRACTICAL

Etaj 13 – 13rd floor

14.30-16.00

Group 1 Instrument settings compensations

 

Group 2 Computer-excercises: Cell viability, doublet exclusion, cell cycle, 4 color labellings

 

 

16.30-17.00

 

Coffee Break

 

 

 

17.00-18.00

Group 1 Instrument settings compensations

 

Group 2 Computer-excercises: Cell viability, doublet exclusion, cell cycle, 4 color labellings

 

 

 

 

 

18.30-18:30

Evaluarea cursului. Concluzii. Inchiderea cursului

 

Course evaluation. Conclusions. Course closing.